Endothelial nitric oxide synthase gene polymorphisms in giant cell arteritis.

نویسندگان

  • Carlo Salvarani
  • Bruno Casali
  • Davide Nicoli
  • Enrico Farnetti
  • Pierluigi Macchioni
  • Maria Grazia Catanoso
  • Qingquan Chen
  • GianLuigi Bajocchi
  • Luigi Boiardi
چکیده

OBJECTIVE To examine potential associations of the Glu/Asp(298) polymorphism in exon 7 and the 4a/b polymorphism in intron 4 of the endothelial nitric oxide synthase (eNOS) gene with susceptibility to and clinical expression of giant cell arteritis (GCA), particularly in patients with versus those without ischemic complications. METHODS Ninety-one consecutive patients with biopsy-proven GCA, who were residents of Reggio Emilia, Italy, and 133 population-based controls from the same geographic area were genotyped by polymerase chain reaction and allele-specific oligonucleotide techniques for eNOS polymorphisms in exon 7 and intron 4. The patients were separated into 2 subgroups according to the presence or absence of ischemic complications (visual loss and/or jaw claudication and/or aortic arch syndrome). RESULTS The distribution of the Glu/Asp(298) genotype differed significantly between GCA patients and controls (corrected P [P(corr)] = 0.003). Carriers of the Asp(298) allele (Asp/Asp or Glu/Asp) were significantly more frequent among the GCA patients than among the controls (P(corr) = 0.0002, odds ratio 3.3, 95% confidence interval 1.7-6.3). The distribution of the 4a/b genotype was similar in GCA patients and controls. No significant associations were found when GCA patients with and without ischemic complications were compared. CONCLUSION Our findings show that the Glu/Asp(298) polymorphism of the eNOS gene is associated with GCA susceptibility.

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عنوان ژورنال:
  • Arthritis and rheumatism

دوره 48 11  شماره 

صفحات  -

تاریخ انتشار 2003